FIGURE 3 | Overexpression of YTHDC2 attenuates radiosensitivity of NPC cell in vitro . (A) Demonstration of YTHDC2 over-expression in CNE2 and HK1 cells. (B) CCK8 assay indicated overexpression of YTHDC2 weaken radiotherapy effect in CNE2 cells. Data are presented as mean ± SD from n = 3. *** P < 0.001, student’s t test. (C) Images of soft agar colony formation assay in CNE2 cells after expose to radiation were shown in 2 different magnifications (40 × and 200 × ) (left) and statistical analysis of colony numbers (50–100 µ m and > 100 µ m) (right). Data are presented as mean ± SD from n = 4. (D) The colonies (left) and survival rate (right) in CNE2 and HK1 cells expose to 0, 2, 4, 6, 8Gy radiation with overexpression of YTHDC2. Data are presented as mean ± SD from n = 3. ** P < 0.01, student’s t- test. (E) The apoptosis assay of CNE2 with overexpression of YTHDC2 after 0 or 6Gy radiation exposure by flow cytometric analysis (left) and data analysis (right). Data are presented as mean ± SD from n = 3, student’s t- test. * P < 0.05, student’s t -test.