FIGURE 2 | Number of nuclei per day 8 blastocyst. Bovine embryos were exposed to standard SOF (A) , control FAF SOF (B), 25 μ M C 18:0 (C) or 25 μ M C 18:1 (D) during day 1 until 5 of in vitro culture. The fi gure presents the number of nuclei per non-expanded (light), expanded (bright) and hatched (dark) blastocyst per group. NucleiwerestainedbyHoechst33342.Asterixindicatesasigni fi cantdifferencebetweentheblastocystsclasseswithinagroup.Forrunsandtotalnumbersee Scheme 1 (in the Materials and Methods section).