Fig 6. Determination of Flag-tagged TRAM0076 co-immunoprecipitated RNAs. (A ) Flowchart of RNA immunoprecipitation experiment. ( B ) qPCR quantification of selected transcripts precipitated by Flag- (0076–3 × Flag) and un-tagged (0076) TRAM0076. Using the precipitated RNAs as templates, qPCR determined the copies of the 5 0 fragments using the primer pairs in the S2 Table. The enrichment percentages by Flag- and un-tagged TRAM0076 were calculated by the method indicated in ( A ). Total RNAs were collected from three batches of cultures, and the average of percent precipitated and standard deviations are shown.