FIGURE3| Lipiddropletnumberandsizeperblastocyst.BovineembryoswereexposedtostandardSOF (A) ,controlFAFSOF (B) ,25 μ MC 18:0 (C) or25 μ MC 18:1 (D) during day 1 until 5 of in vitro culture. Panel A – D shows representative images for the different groups with in blue DNA (Hoechst 33342) and in green lipid droplets (LD540), Bar = 50 µm. Lipid droplet number (E) , size (F) and total lipid in μ m 2 (G) are shown for blastocysts of each group. Different superscripts differ signi fi cantly. For runs and total number see Scheme 1 (in the Materials and Methods section).