Figure 5. Summary of zebrafish tert mutant analysis. ( A ) Workflow for the analysis of zebrafish tert mutants. ( B ) Differen- Figure 5. Summary of zebrafish tert mutant analysis. ( A ) Workflow for the analysis of zebrafish tert mutants. ( B ) Differen- tially expressed gene (DEG) (left panels) and gene set enrichment (GSE) analysis (right panels) of zebrafish brain samples. tially expressed gene (DEG) (left panels) and gene set enrichment (GSE) analysis (right panels) of zebrafish brain samples. DEG and GSE analyses were performed on zebrafish tert mutant brain expression data for tert − / − (upper panels) and DEG and GSE analyses were performed on zebrafish tert mutant brain expression data for tert −/− (upper panels) and tert +/− tert +/ − (lower panels), using tert +/+ as a reference. Methods and colour keys are as in Figure 2. For muscle, liver, skin, and (lower panels), using tert +/+ as a reference. Methods and colour keys are as in Figure 2. For muscle, liver, skin, and pseudo- pseudo-whole animal analyses, refer to Figure S3. For full data, refer to Supplementary Data S5. ( C ) Reporter metabolite whole animal analyses, refer to Figure S3. For full data, refer to Supplementary Data 5. ( C ) Reporter metabolite analysis analysis of zebrafish samples. DEG data were overlaid on ZebraGEM2.1 to determine reporter metabolites. Shown are of zebrafish samples. DEG data were overlaid on ZebraGEM2.1 to determine reporter metabolites. Shown are reporter reporter metabolites with p < 0.1 within the retinoic acid metabolic pathway. Red numbers indicate p -values in tert − / − metabolites with p < 0.1 within the retinoic acid metabolic pathway. Red numbers indicate p -values in tert −/− compared to compared to tert +/+ . Blue numbers indicate p -values in tert +/ − compared to tert +/+ . Green numbers indicate p -values in tert +/+ . Blue numbers indicate p -values in tert +/- compared to tert +/+ . Green numbers indicate p -values in tert −/− compared to tert − / − compared to tert +/ − . Tissues are indicated with icons. For full data, refer to Supplementary Data tert +/− . Tissues are indicated with icons. For full data, refer to Supplementary Data 6.