Fig 4. Histological changes in the skeletal muscle. Gastrocnemius muscle wet weight ( A ), the ratio of muscle wet weight to body weight ( B ), representative gastrocnemius lateralis images ( C ), and cross-sectional area of each muscle fiber type ( D ). wo, week-old; BW, body weight; SDH, succinate dehydrogenase. I, IIA, and IIB denote type I, IIA, and IIB fibers, respectively. Data are illustrated as mean ± SEM. � and † show significantly different from LETO rats and the OLETF Sed group, respectively, P < 0.05, one way ANOVA. n = 5–6 per group. https://doi.org/10.1371/journal.pone.0270330.g004