Figure 2: Morphology of BV-2 cells upon 24 h of LPS stimulation and RBV treatment. Phalloidin/Hoechst fluorescent staining (red/blue) of control (a), LPS (b), and LPS stimulated BV-2 cells treated with 0.1 (c), 1 (d), and 10 𝜇 M RBV (e). (f) Cell surface area was measured using AxioVision Rel. 4.6 software, in five areas (138 × 104 𝜇 m 2 ) per each coverslip ( 𝑛 = 3 ) per experimental group in three independent experiments. Bars represent mean surface areas ( ± SEM) obtained from data presented in (a)–(e). ∗ 𝑃 < 0.05 versus control group, # 𝑃 < 0.05 versus LPS stimulated group. Scale bar (a)–(e): 50 𝜇