FIGURE 5 Effects of WSP on SGC-7901 migration after EGFRVIII knockdown. (A) SGC-7901 cells under bright fi eld, green fl uorescence fi eld and merge after 48h siRNA transfection. (B) Western blotting analysis of EGFRVIII in SGC-7901 and sh-SGC-7901 after siRNA – EGFRVIII transfection. (C) qPCR analysis of EGFRVIII in SGC-7901 and sh-SGC-7901 after siRNA EGFRVIII transfection. (D) Western blotting analysis of AKT, PI3K, MMP2, and MMP9 in SGC-7901 or sh-SGC-7901 treated without or with WSP. (E) Qualitative of cancer number migration in SGC-7901 and sh- SGC-7901 cells treated without or with WSP as shown in (E) . (F) Quanti fi cation of cell number migration in SGC-7901 and sh-SGC-7901 cells treated without or with WSP as shown in (F) . *P < 0.05, ***P < 0.005, ns, no statistical