Figure 6 . (C,D) Representative images of western blot analysis. (C) Hsp70 protein level in the head of 10-day-old HTT (elav > Q20) or mHTT expressing (elav > Q93) adult females with RNAi silencing adoR and mod(mdg4) . (D) The Hsp70 protein level was quantified by normalizing the intensity of the Hsp70 band to the α -tubulin band by using ImageJ; values of each treatment group were further normalized to the elav-gal4 control. Significance was analyzed by Student’s t -test; significant differences between HTT-expressing flies (elav > Q20) and each RNAi treatment of Q93-expressing flies are labeled as follows: * P < 0.05, ** P < 0.01, *** P < 0.001. n = 4. Error bars are presented as mean ± SEM. Original gel images are presented in the Supplementary Figure 7 . (E) Survival of w 1118 and homozygous adoR mutant adult males after paraquat (PQ) injection. Control groups were injected with ringer buffer. Significance was analyzed by weighted