Figure 5. Microdilution MIC test with naringin against MSSA, MRSA, and VRSA. ( A ) S. aureus Figure 5. Microdilution MIC test with naringin against MSSA, MRSA, and VRSA. ( A ) S. aureus ATCC ATCC 6538 (MSSA), ( B ) S. aureus ATCC 33591 (MRSA), ( C ) S. aureus CCARM 3795 (MRSA), and ( D ) 6538 (MSSA), ( B ) S. aureus ATCC 33591 (MRSA), ( C ) S. aureus CCARM 3795 (MRSA), and ( D ) VRSA48. VRSA48. P is a positive control without any antimicrobial agents, and N is a negative control with P is a positive control without any antimicrobial agents, and N is a negative control with only media only media and DW. In the 96-well plate, from left to right, the GSE and pH 76% G (naringin) con- and DW. In the 96-well plate, from left to right, the GSE and pH 76% G (naringin) concentrations centrations were 1/2 to 1/1024, and the concentrations of antibiotics were 32 to 0.06 µg/mL. The MICs were 1/2 to 1/1024, and the concentrations of antibiotics were 32 to 0.06 µ g/mL. The MICs of pH of pH 76% G (naringin) are indicated by red arrows, and antibiotic MICs are indicated by blue ar- 76% G (naringin) are indicated by red arrows, and antibiotic MICs are indicated by blue arrows. rows. MIC values <1/1024 or >32 µg/mL are not indicated by arrows. The MIC values for GSE, pH MIC values <1/1024 or >32 µ g/mL are not indicated by arrows. The MIC values for GSE, pH 76% G 76% G (naringin), and antibiotics for each of the bacterial strains are also illustrated by bar graphs (naringin), and antibiotics for each of the bacterial strains are also illustrated by bar graphs next to next to the 96-well plate figures. In the bar graphs, the MIC values of GSE and pH 76% G (naringin) can be read along the left y -axis indicated by the red arrow, and the MIC values of antibiotics can the 96-well plate figures. In the bar graphs, the MIC values of GSE and pH 76% G (naringin) can be be read along the right y -axis indicated by the blue arrow. GSE and the antibiotics showed the same read along the left y -axis indicated by the red arrow, and the MIC values of antibiotics can be read effect, as shown in Figure 4. The pH 76% G (naringin) did not show a better effect than the pH 76% along the right y -axis indicated by the blue arrow. GSE and the antibiotics showed the same effect, G. This indicates that substances other than naringin in GSE affect the antibacterial activity of GSE. as shown in Figure 4. The pH 76% G (naringin) did not show a better effect than the pH 76% G. This indicates that substances other than naringin in GSE affect the antibacterial activity of