FIGURE6 Effect of GABA-A receptor antagonist on network function. GABA-A receptors were blocked by gabazine in two HD-MEAs on 120 dpi. Baseline activity, activity under gabazine treatment and activity after washout were recorded. (A) Signal traces recorded from two electrodes at baseline, under gabazine and after washout. (B) Raster plot of activity in individual electrodes (left) and 10 selected neurons (right) in the three different conditions with each row representing an electrode or neuron. (C) AP and burst frequency, burst duration and percentage of APs appearing in burst events were compared between the three conditions using Kruskal-Wallis test followed by Dunn’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001, and ns, non-significant. Each point represents the corresponding activity feature in one