FIGURE5 Effect of AMPA and NMDA receptor antagonists on network function. AMPA and NMDA receptors were blocked by NBQX+APV in two HD-MEAs on 118 dpi. Activity was recorded and analyzed for 5 min in the baseline, treatment and washout conditions. (A) Signal traces in two electrodes at baseline, under NBQX+APV treatment and washout. (B) Ten second raster plot of activity in all active electrodes (left) and 10 selected neurons (right) in the three different conditions with each row representing an electrode or neuron. (C) AP and burst frequency, burst duration and percentage of APs appearing in burst events were compared between the three conditions using Kruskal-Wallis test followed by Dunn’s multiple comparison test. ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001. Each point represents the corresponding activity feature in one