Figure 2. Akt inhibitor enhances viral proteins and the generation of infectious progeny virus. A549 cells were infected with clinical isolate NH409A and subsequently treated with varying concentrations of MK-2206, an Akt inhibitor. Protein analyses and the generation of infectious progeny virus was measured at 24 h post infection (m.o.i = 0.2). Asterisk ( * ) or non-significant (ns) is compared to vehicle (Veh) control. ( A ) Western blot of cellular and viral proteins. The 2 forms of S6 are designated by the red and black arrows. ( B – F ) Quantification of cellular and viral proteins displayed in A (n = 2). ( B ) phospho-Akt/Akt; ( C ) phospho-S6K1/S6K1; ( D ) total ribosomal protein S6/β-actin; ( E ) RSV nucleoprotein N/β-actin; ( F ) RSV fusion protein F1/β-actin. ( G ) Quantification of virus progeny in the absence and presence of MK-2206 (n = 3). Error bars, SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; n.s.