Figure 4: Rotenone treatment blunted inflammatory response in obstructed kidneys. (a) qRT-PCR analysis of TNF- 𝛼 , IL-1 𝛽 , and ICAM-1 mRNA expressions. (b) ELISA analysis of renal TNF- 𝛼 protein level. (c) ELISA analysis of renal IL-1 𝛽 protein level. 𝑁 = 5 in each group. Data are means ±