FIGURE 3 | ESR1 expression is negatively correlated with CRC cell sensitivity to 5-FU. ESR1 was up-regulated in (A) HCT116 and (B) KM12 cells, which were treated with increasing concentrations of 5-FU after transfection with ESR1 or control. Overexpression ESR1 decreases sensitivity to 5-FU. ESR1 was knocked down in (C) DLD1 cell and (D) sw480 cell, which were treated with increasing concentrations of 5-FU after transfection with ESR1 or control. Knocking down ESR1 gives rise to higher sensitivity to 5-FU. (E) KM12 and ESR1 overexpressing HCT116 and KM12 cells displayed increased activation of P65 and ESR1 knocking down DLD1 and sw480 cells displayed decreased activation of P65. * p < 0.05, ** p <