Fig 6. Effect of delaying re-infection on the level of circulating murine IFN- γ + T cells after challenge. (A) Timeline of experiment. Infected BALB/c mice were cured with benznidazole treatment, which was initiated 36 days post- infection, as in Fig 5. They were re-infected 338 days later, and blood samples collected on the days indicated. (B) PBMCs were isolated from re-infected mice at various time points pre and post re-infection. IFN- γ + PBMCs were quantified after overnight stimulation with the 20-mer ASP-2/TS peptide pool. ( � ) p = 0.024.