Fig 9. Reduction in IFN- β gene transcription correlates with cleavage and/or degradation of RLR signaling proteins. (A+B) Hela R19 cells were infected at MOI 10 with the indicated viruses and cells were lysed at 8 hpi. Total RNA was isolated and used for RT-qPCR analysis for IFN- β and actin mRNA (upper panels), and EMCV vRNA (lower panels). The IFN- β levels are depicted as a fold induction compared to levels in mock-infected cells, after correction for actin mRNA levels. The EMCV vRNA is depicted as a copy number per cell, calculated from a plasmid standard. Error bars represent the SD. One-way ANOVA with the Dunnet post hoc test was used to determine statistical significance compared to the results for EMCV-L Zn -infected cells ( �� , p < 0.01; ��� , p < 0.001; ���� , p <