Fig 5. Mutation of L143 or SAP domain strongly reduces cleavage and/or degradation of RLR signaling proteins. HeLa R19 cells were infected at MOI 10 with the indicated viruses and lysed at 2, 4, 6, 8 h pi. Cell lysates were subjected to Western Blot analysis for MAVS, TBK1, NF- κ B p65, IRF3, eIF4G, G3BP1, L pro , EMCV capsid proteins and tubulin. https://doi.org/10.1371/journal.ppat.1008702.g005