Fig 1. Immunogenicity of the recombinant ASP-2/TS vaccine. (A) Open reading frame encoding ASP-2 and TS peptides, with the mouse Ig kappa chain signal peptide and furin 2A splice site linker indicated. (B) Prime-boost vaccination strategy. BALB/c mice (n = 5) were inoculated one week apart with ChAdOx1 (priming) and then MVA (boosting) recombinant vaccines containing the ASP-2/TS fusion gene. Control mice received vaccinations with the same viral vectors engineered to express dengue protein NS-1 (n = 3). (C) ELISpot analysis. Splenocytes were plated onto antibody coated ELISpot plates and stimulated for 16 h with a peptide pool covering the entire ASP-2 and TS sequences (Materials and Methods). Data are presented as ex vivo IFN- γ spot forming cells (SFCs) per 10 6