Figure 2. Effects of ECE and dieckol on lipid droplets (LDs) modulation in the muscle. ( A ) The images of low and high Figure 2. Effects of ECE and dieckol on lipid droplets (LDs) modulation in the muscle. ( A ) The images of low and high magnification show the myosin type I, IIa or IIb with oil red O staining. ( B ) The average size of LDs in the muscle was magnification show the myosin type I, IIa or IIb with oil red O staining. ( B ) The average size of LDs in the muscle was increased by HF/saline and decreased after treatment with ECE or dieckol. ( C ) The numbers of LDs in type I fiber were increased by HF/saline and decreased after treatment with ECE or dieckol. ( C ) The numbers of LDs in type I fiber were increased by ECE or dieckol treatment. ( D ) The numbers of LDs in type II fiber were increased by HF/saline and were increased by ECE or dieckol treatment. ( D ) The numbers of LDs in type II fiber were increased by HF/saline and were decreased after treatment with ECE or dieckol. ( E ) The number of LDs in the SS muscle was increased by HF/saline and decreased after treatment with ECE or dieckol. ( E ) The number of LDs in the SS muscle was increased by HF/saline and decreased after treatment with ECE or dieckol. ( F ) The number of LDs in the IMF muscle was decreased by HF/saline and decreased after treatment with ECE or dieckol. ( F ) The number of LDs in the IMF muscle was decreased by HF/saline and increased after treatment with ECE or dieckol. Scale bar = 100 µm. Data are presented as mean ± SD. **, p < 0.01 and ***, p increased after treatment with ECE or dieckol. Scale bar = 100 µ m. Data are presented as mean ± SD. **, p < 0.01 and ***, < 0.001 vs. Chow/saline; $$, p < 0.01 vs. HF/saline; #, p < 0.05 and ##, p < 0.01 vs. HF/ECE100 (Mann–Whitney U test). conc., p < 0.001 vs. Chow/saline; $$, p < 0.01 vs. HF/saline; #, p < 0.05 and ##, p < 0.01 vs. HF/ECE100 (Mann–Whitney U