Figure 1. Rapamycin enhances viral protein expression and generation of progeny virus. A549 cells were infected with clinical isolate NH409A and subsequently treated with varying concentrations of rapamycin. Protein analyses and production of progeny virus was measured at 24 h post infection (m.o.i = 0.2). Asterisk ( * ) or non-significant (ns) is compared to vehicle (Veh) control. ( A ) Western blot of cellular and viral proteins. The 2 forms of S6 are designated by the red and black arrows. ( B – F ) Quantification of cellular and viral proteins displayed in ( A ) (n = 3). ( B ) phospho-Akt/Akt; ( C ) phospho-S6K1/S6K1; ( D ) total ribosomal protein S6/β- actin; ( E ) RSV nucleoprotein N/β-actin; ( F ) RSV fusion protein F1/β-actin. ( G ) Quantification of virus progeny in the absence and presence of rapamycin (n = 3). Error bars, SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; n.s.