Figure 5: Rotenone treatment reduced protein expression of fibrotic markers in obstructed kidneys. (a) Immunohistochemistry of collagen I. (b) Fibrotic markers of collagen I, FN, and 𝛼 -SMA were determined by Western blotting, and 𝛽 -actin was used as loading control. (c) Densitometry of Western blots. 𝑁 = 5 in each group. Data are means ±