Figure 2. Catalytic sites in FKP and functional analysis of FKP . (A) Catalytic sites in FKP. Two close-up views of the active sites are shown on the right. The GTP binding sites are colored in salmon on the top panel. The fucose binding sites are colored in blue and the ATP binding sites are colored in salmon on the bottom panel. (B) The single point mutation reduced the activities of FKP, fucokinase activity or GDP-fucose pyrophosphorylase activity. The relative activity of each reaction was normalized to that of the reaction containing substrate and FKP (100%). Each reaction was repeated at least three times. Error bars represent