FIGURE1 Seeding iNGN-EGFP cells in two step and supporting them with astrocyte-conditioned medium. iNGN-EGFP cells were differentiated for 4 days on a Matrigel coated plate. Ara-C was applied on 3 dpi to remove undifferentiated cells. On 4 dpi iNGN-EGFP cells were dissociated and seeded on a PDL-laminin coated HD-MEA chip. Parallel astrocyte culture on PDL-laminin coated glass coverslips was used to provide astrocyte-conditioned medium for neuronal