FIGURE 4 | Knockout of miR-29a exhibited M1 polarization of microglia in rat brain. (A,B) Western blot detection of microglia M1 and M2 marker changes in the brains of wild-type rats and miR-29a-5p knockout rats. (A) CD206, iNOS (B) Arg1, CD16. (C – F) Bar graphs of marker changes in the brains of wild-type rats and miR- 29a knockout rats. (C) CD206, (D) Arg1, (E) CD16, (F) iNOS. (G,H) Representative double immuno fl uorescence staining for CD206 (green) or iNOS (green), and Iba-1 (red) markers. (G) CD206, (H) iNOS. * p < 0.05. Arg1, arginase 1; iNOS, inducible nitric oxide synthase.