Here is the design of a clinical trial:

Official Title: Effect of Targeted vs Standard Fortification of Breast Milk on Growth and Development of Preterm Infants (≤ 32 Weeks): a Randomised Controlled Trial

Included conditions:
- BPD - Bronchopulmonary Dysplasia
- NEC - Necrotizing Enterocolitis
- Weight Gain
- VLBW - Very Low Birth Weight Infant
- ROP - Retinopathy of Prematurity

Study Armgroups:
- {'label': 'tailored enteral nutrition', 'type': 'EXPERIMENTAL', 'description': 'Tailored Human milk fortification procedure Tailored milk fortification will be done twice a day (8 am and 8 pm) for each following 12 hour nursing shift. Standard fortification will be added first. The remainder amount of protein, lipids and carbohydrates required to meet the recommended by ESPGHAN doses will be acheived by adding single ingrediant nutrients.', 'interventionNames': ['Dietary Supplement: Tailored enteral nutrition']}
- {'label': 'standard enteral nutrition', 'type': 'NO_INTERVENTION', 'description': 'Standard fortification will be added according to the unit protocol.'}

Interventions:
- {'type': 'DIETARY_SUPPLEMENT', 'name': 'Tailored enteral nutrition', 'description': 'The mean of three measurements per batch (3 x 2-3ml) will be used to calculate the required amount of extra fat, protein, and carbohydrate for the following 3 days of fortification using a predefined Excel spread sheet (Microsoft Inc, Redmond, Washington).\n\nThe intervention will consist of adding fat, protein, and/or carbohydrate to achieve target levels of macronutrients.\n\nThe defined macronutrient concentration in breast milk is 4.4 g/100 mL of fat, 3 g/100 mL of protein, and 8.8 g/ 100 mL of carbohydrate to meet the European Society for Paediatric Gastroenterology, Hepatology, and Nutrition (ESPGHAN) guidelines (6.6 g/kg/d of fat, 4.5 g/kg/d of protein, and 13.2 g/kg/d of carbohydrate) assuming an intake of 150 mL/kg/d.', 'armGroupLabels': ['tailored enteral nutrition']}

Primary Outcomes:
- {'measure': 'Growth', 'description': 'weight will be assessed every day.', 'timeFrame': 'from birth at postmentrual age <32 to up to 37 weeks of post-conceptional age'}
- {'measure': 'Velocity of weight gain in grams', 'description': 'weight will be assessed every day.', 'timeFrame': 'from birth at postmentrual age <32 to up to 37 weeks of post-conceptional age'}

Please estimate the sample size based on the assumption: 
Type I error (α) is 5%, power is 80%, equal sample sizes in both groups, and the standard deviation for weight gain is increased by 50% to 3.75. For the secondary outcome, the mean cognitive score is 100 points with a standard deviation of 12. An attrition rate of 20% is assumed for weight gain, and 10% for the overall study.
Sample size {14}
        The sample size required to compare two means in two-sided equality test was estimated based on results from a prior double blind, randomized clinical trial, investigating the effect of TG vs SF of breast milk on the changes of anthropometric parameters and body composition in preterm children [23, 24]. It was determined that a mean difference of weight gain 1.9âg/kg/day between groups would be clinically important and feasible during intervention.
        The following assumptions were made for the calculation: type I error (Î±) 5%, power 80%, equal sample sizes in both groups, the mean weight gain in the standard fortification group 19.3âg/kg/day, and the mean weight gain in the target fortification group 21.2âg/kg/day. To account for the higher uncertainty in measured weight gain due to differences between the studied and the quoted trial population, standard deviation value taken from the prior trial was increased by 50% to 3.75.
        An estimated minimum size of each group is 68. Accounting for a presumed 20% attrition rate, due to potential dropouts, deviations from the protocol, and loss to follow-up, a minimum sample size required was estimated at 156 infants or 78 infants per treatment arm.
        However, in order to detect a difference of 5 points in cognitive score of Bayley-III (secondary outcome) at 12âmonths of age (mean of 100 points, standard deviation (SD) 12) between the study groups with a power of 80% and Î±â=â0.05, we have decided to increase the sample to 91 infants in each study group.
        In summary, allowing for 10% of loss to follow-up, the target number of 200 premature infants will be recruited.