Here is the design of a clinical trial:

Official Title: A Double Blind, Randomized Controlled Pilot Trial of Gargling Agents in Reducing Intraoral Viral Load Among Laboratory Confirmed COVID-19 Patients: GARGLES STUDY

Included conditions:
- Covid-19

Study Armgroups:
- {'label': 'Povidone-Iodine 0.2% (BETADINE®)', 'type': 'EXPERIMENTAL', 'description': '0.2% Povidone-Iodine (BETADINE®) 10 ml gargle and nasal lavage for 20-30 seconds, thrice daily for 6 days.', 'interventionNames': ['Drug: Gargle/Mouthwash']}
- {'label': 'Hydrogen peroxide 1% (ActiveOxy)', 'type': 'EXPERIMENTAL', 'description': 'ActiveOxy (1% Hydrogen peroxide) 10 ml gargle and nasal lavage for 20-30 seconds, thrice daily for 6 days.', 'interventionNames': ['Drug: Gargle/Mouthwash']}
- {'label': 'Neem extract (Azadirachta indicia)', 'type': 'ACTIVE_COMPARATOR', 'description': 'Neem extract (Azadirachta indicia) gargle will be prepared by chemistry laboratory. patients will do 10ml gargle and nasal lavage for 20-30 seconds, thrice daily for 6 days.', 'interventionNames': ['Drug: Gargle/Mouthwash']}
- {'label': 'Hypertonic saline (2%NaCl)', 'type': 'ACTIVE_COMPARATOR', 'description': '10 ml gargle and nasal lavage using Hypertonic saline for 20-30 seconds, thrice daily for 6 days.', 'interventionNames': ['Drug: Gargle/Mouthwash']}
- {'label': 'Positive controls', 'type': 'PLACEBO_COMPARATOR', 'description': '10 ml gargle and nasal lavage using distilled water for 20-30 seconds, thrice daily for 6 days.', 'interventionNames': ['Drug: Gargle/Mouthwash']}

Interventions:
- {'type': 'DRUG', 'name': 'Gargle/Mouthwash', 'description': 'There will be 50 patients in six study groups. Group A (n=10) patients on 10 ml gargle and nasal lavage using 0.2% Povidone-Iodine (Betadiene®) for 20-30 seconds, thrice daily for 6 days.\n\nGroup B (n=10) patients will be subjected to 10 ml gargle and nasal lavage using 1% Hydrogen peroxide (ActiveOxy®) for 20-30 seconds, thrice daily for 6 days.\n\nGroup C will comprised of (n=10) subjects on 10ml gargle and nasal lavage using Neem extract solution (Azardirachta indica) formulated locally) for 20-30 seconds, thrice daily for 6 days.\n\nGroup D (n=10) patients will use 2% hypertonic saline (Plabottle®) gargle and nasal lavage for a similar time period.\n\nGroup E (n=10) will serve as positive controls. These will be given simple distilled water gargles and nasal lavage for 20-30 seconds, thrice daily for six days Whereas Group F (n=5) will comprise of negative controls, who will not use any gargles or nasal lavage during study period.', 'armGroupLabels': ['Hydrogen peroxide 1% (ActiveOxy)', 'Hypertonic saline (2%NaCl)', 'Neem extract (Azadirachta indicia)', 'Positive controls', 'Povidone-Iodine 0.2% (BETADINE®)'], 'otherNames': ['Gargling agent', 'Mouthrinse']}

Primary Outcomes:
- {'measure': 'Intraoral viral load', 'description': 'Intraoral viral load as deciphered by RT-PCR', 'timeFrame': 'Five days of using gargles'}

Please estimate the sample size based on the assumption: 
The present study will serve as a pilot trial.
Numbers to be randomised (sample size)
          As there is no prior work on this research question, so no assumptions for the sample size calculation could be made. The present study will serve as a pilot trial. We intend to study 50 patients in five study groups with 10 patients in each study group. For details, please refer to Fig.Â 1 for details.