Upload 21 files
Browse files- .gitattributes +10 -0
- H3K27me3/H3K27me3.csv +3 -0
- H3K27me3/H3K27me3_test.csv +0 -0
- H3K27me3/H3K27me3_train.csv +3 -0
- H3K27me3/H3K27me3_val.csv +0 -0
- H3K4me1/H3K4me1.csv +3 -0
- H3K4me1/H3K4me1_test.csv +0 -0
- H3K4me1/H3K4me1_train.csv +3 -0
- H3K4me1/H3K4me1_val.csv +0 -0
- H3K4me3/H3K4me3.csv +3 -0
- H3K4me3/H3K4me3_test.csv +0 -0
- H3K4me3/H3K4me3_train.csv +3 -0
- H3K4me3/H3K4me3_val.csv +0 -0
- H3K9me3/H3K9me3.csv +3 -0
- H3K9me3/H3K9me3_test.csv +0 -0
- H3K9me3/H3K9me3_train.csv +3 -0
- H3K9me3/H3K9me3_val.csv +0 -0
- H3k27ac/H3k27ac.csv +3 -0
- H3k27ac/H3k27ac_test.csv +0 -0
- H3k27ac/H3k27ac_train.csv +3 -0
- H3k27ac/H3k27ac_val.csv +0 -0
- README.md +137 -3
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README.md
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-
---
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license: cc-by-nc-4.0
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---
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license: cc-by-nc-4.0
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task_categories:
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- text-classification
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language:
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- en
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tags:
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- genomics
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- fish
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- DNA
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- benchmark
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- promoter
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- splice-site
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- transcription-factor-binding-site
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- histone-mark
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size_categories:
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- 100K<n<1M
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pretty_name: FishGUE
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---
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# FishGUE
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**FishGUE** is a unified fish genomics benchmark developed for evaluating DNA foundation models and other sequence models on fish genomic prediction tasks.
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It was introduced in the FishNALM study, where it was used to systematically benchmark fish-specific and general DNA language models across diverse regulatory sequence prediction tasks.
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## Dataset summary
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FishGUE contains **17 supervised prediction tasks** spanning four task groups:
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- **Histone mark prediction**
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- **Transcription factor binding-site prediction**
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- **Promoter prediction**
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- **Splice-site prediction**
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The benchmark covers input sequence lengths from **300 bp to 2500 bp** and includes both **zebrafish-based datasets** and **multi-species cyprinid splice datasets**.
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## Why FishGUE?
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Fish genomic benchmarks remain limited compared with those available for human, plant, or broad multi-species genomic modeling. FishGUE was constructed to provide a unified benchmark for evaluating model performance on representative fish genomic sequence prediction problems, especially those relevant to regulatory sequence recognition and gene structure annotation.
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## Benchmark composition
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The FishGUE benchmark includes the following 17 tasks.
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| Category | Task | Species | Sequence length (bp) | Train / Val / Test |
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|---|---|---|---:|---:|
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| Histone mark prediction | H3K4me1 | *Danio rerio* | variable (≤2500) | 48815 / 6102 / 6102 |
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| 49 |
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| Histone mark prediction | H3K4me3 | *Danio rerio* | variable (≤2500) | 47482 / 5935 / 5936 |
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| 50 |
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| Histone mark prediction | H3K9me3 | *Danio rerio* | variable (≤2500) | 40125 / 5016 / 5016 |
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| 51 |
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| Histone mark prediction | H3K27ac | *Danio rerio* | variable (≤2500) | 27611 / 3451 / 3452 |
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| 52 |
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| Histone mark prediction | H3K27me3 | *Danio rerio* | variable (≤2500) | 27017 / 3377 / 3378 |
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| 53 |
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| TF binding-site prediction | CTCF | *Danio rerio* | 800 | 28526 / 3566 / 3566 |
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| 54 |
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| TF binding-site prediction | Pou5f1 | *Danio rerio* | 800 | 11128 / 1391 / 1391 |
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| 55 |
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| TF binding-site prediction | Sox2 | *Danio rerio* | 800 | 9717 / 1215 / 1215 |
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| 56 |
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| Promoter prediction | Core promoter | *Danio rerio* | 300 | 17222 / 2153 / 2153 |
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| 57 |
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| Promoter prediction | Core promoter (TATA) | *Danio rerio* | 300 | 3464 / 433 / 434 |
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| 58 |
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| Promoter prediction | Core promoter (non-TATA) | *Danio rerio* | 300 | 13757 / 1720 / 1720 |
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| 59 |
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| Promoter prediction | Promoter | *Danio rerio* | 1000 | 17222 / 2153 / 2153 |
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| 60 |
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| Promoter prediction | Promoter (TATA) | *Danio rerio* | 1000 | 3464 / 433 / 434 |
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| 61 |
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| Promoter prediction | Promoter (non-TATA) | *Danio rerio* | 1000 | 13757 / 1720 / 1720 |
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| 62 |
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| Splice-site prediction | Splicing (acceptor) | Cyprinidae (5 species) | 600 | 17200 / 2150 / 2150 |
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| 63 |
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| Splice-site prediction | Splicing (donor) | Cyprinidae (5 species) | 600 | 27600 / 3450 / 3450 |
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| 64 |
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| Splice-site prediction | Splicing (both) | Cyprinidae (5 species) | 600 | 1720 / 2150 / 2105 |
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| 65 |
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## Data sources and construction overview
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According to the FishNALM manuscript, FishGUE was assembled from the following sources:
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- **Histone mark datasets** were derived from public zebrafish ChIP-seq peak datasets.
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- **Transcription factor binding-site datasets** were built from public ChIP-seq peaks for **CTCF**, **Pou5f1**, and **Sox2**.
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- **Promoter datasets** were constructed from zebrafish promoter annotations from the **Eukaryotic Promoter Database (EPD)**.
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- **Splice-site datasets** were compiled from annotated genomes of **five cyprinid fish species**.
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For all tasks, positive and negative examples were constructed through task-specific processing, then split into **training**, **validation**, and **test** sets using an **8:1:1** ratio.
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## Suggested repository organization
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| 78 |
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If you plan to upload FishGUE as a single Hugging Face dataset repository, a clean structure is:
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```text
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| 82 |
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FishGUE/
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├── README.md
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├── histone/
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│ ├── H3K4me1_train.tsv
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│ ├── H3K4me1_val.tsv
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│ ├── H3K4me1_test.tsv
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│ └── ...
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├── tfbs/
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│ ├── CTCF_train.tsv
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│ ├── CTCF_val.tsv
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│ ├── CTCF_test.tsv
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│ └── ...
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├── promoter/
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│ ├── core_promoter_300_train.tsv
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│ └── ...
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└── splice/
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├── splice_acceptor_train.tsv
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└── ...
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```
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If your files are already packaged in another layout, you can keep that layout and simply explain it in this README.
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## Intended uses
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FishGUE is intended for:
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- benchmarking DNA foundation models on fish genomic sequence prediction tasks
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- evaluating transfer learning performance in fish genomics
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- comparing task robustness across promoter, chromatin, TFBS, and splice-site prediction settings
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- developing new fish-specific sequence models and downstream classifiers
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## Limitations
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| 114 |
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| 115 |
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- Most non-splice tasks are based on **zebrafish** data.
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| 116 |
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- The splice-site benchmark uses **five cyprinid species**, so broader phylogenetic coverage remains limited.
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| 117 |
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- FishGUE is designed as a **research benchmark** and should not be interpreted as a clinical or diagnostic dataset.
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| 118 |
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- Performance on FishGUE does not guarantee performance on all fish species or all regulatory genomics tasks.
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| 119 |
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## Citation
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| 121 |
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| 122 |
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If you use FishGUE in your work, please cite the FishNALM manuscript.
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| 123 |
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**Manuscript:**
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| 125 |
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| 126 |
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> FishNALM: A Foundation DNA Language Model for Fish Genomes
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| 127 |
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| 128 |
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## Project links
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| 129 |
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- **GitHub**: [bioinfoihb/FishNALM](https://github.com/bioinfoihb/FishNALM)
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| 131 |
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| 132 |
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## Contact
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| 133 |
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| 134 |
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**Xiao-Qin Xia**
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Institute of Hydrobiology, Chinese Academy of Sciences
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Email: xqxia@ihb.ac.cn
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Email: bioinfoihb@ihb.ac.cn
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