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- ---
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- license: cc-by-nc-4.0
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- ---
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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+ ---
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+ license: cc-by-nc-4.0
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+ task_categories:
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+ - text-classification
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+ language:
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+ - en
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+ tags:
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+ - genomics
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+ - fish
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+ - DNA
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+ - benchmark
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+ - promoter
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+ - splice-site
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+ - transcription-factor-binding-site
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+ - histone-mark
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+ size_categories:
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+ - 100K<n<1M
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+ pretty_name: FishGUE
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+ ---
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+
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+ # FishGUE
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+
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+ **FishGUE** is a unified fish genomics benchmark developed for evaluating DNA foundation models and other sequence models on fish genomic prediction tasks.
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+
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+ It was introduced in the FishNALM study, where it was used to systematically benchmark fish-specific and general DNA language models across diverse regulatory sequence prediction tasks.
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+
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+ ## Dataset summary
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+
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+ FishGUE contains **17 supervised prediction tasks** spanning four task groups:
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+
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+ - **Histone mark prediction**
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+ - **Transcription factor binding-site prediction**
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+ - **Promoter prediction**
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+ - **Splice-site prediction**
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+
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+ The benchmark covers input sequence lengths from **300 bp to 2500 bp** and includes both **zebrafish-based datasets** and **multi-species cyprinid splice datasets**.
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+
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+ ## Why FishGUE?
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+
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+ Fish genomic benchmarks remain limited compared with those available for human, plant, or broad multi-species genomic modeling. FishGUE was constructed to provide a unified benchmark for evaluating model performance on representative fish genomic sequence prediction problems, especially those relevant to regulatory sequence recognition and gene structure annotation.
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+
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+ ## Benchmark composition
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+
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+ The FishGUE benchmark includes the following 17 tasks.
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+
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+ | Category | Task | Species | Sequence length (bp) | Train / Val / Test |
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+ |---|---|---|---:|---:|
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+ | Histone mark prediction | H3K4me1 | *Danio rerio* | variable (≤2500) | 48815 / 6102 / 6102 |
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+ | Histone mark prediction | H3K4me3 | *Danio rerio* | variable (≤2500) | 47482 / 5935 / 5936 |
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+ | Histone mark prediction | H3K9me3 | *Danio rerio* | variable (≤2500) | 40125 / 5016 / 5016 |
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+ | Histone mark prediction | H3K27ac | *Danio rerio* | variable (≤2500) | 27611 / 3451 / 3452 |
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+ | Histone mark prediction | H3K27me3 | *Danio rerio* | variable (≤2500) | 27017 / 3377 / 3378 |
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+ | TF binding-site prediction | CTCF | *Danio rerio* | 800 | 28526 / 3566 / 3566 |
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+ | TF binding-site prediction | Pou5f1 | *Danio rerio* | 800 | 11128 / 1391 / 1391 |
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+ | TF binding-site prediction | Sox2 | *Danio rerio* | 800 | 9717 / 1215 / 1215 |
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+ | Promoter prediction | Core promoter | *Danio rerio* | 300 | 17222 / 2153 / 2153 |
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+ | Promoter prediction | Core promoter (TATA) | *Danio rerio* | 300 | 3464 / 433 / 434 |
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+ | Promoter prediction | Core promoter (non-TATA) | *Danio rerio* | 300 | 13757 / 1720 / 1720 |
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+ | Promoter prediction | Promoter | *Danio rerio* | 1000 | 17222 / 2153 / 2153 |
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+ | Promoter prediction | Promoter (TATA) | *Danio rerio* | 1000 | 3464 / 433 / 434 |
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+ | Promoter prediction | Promoter (non-TATA) | *Danio rerio* | 1000 | 13757 / 1720 / 1720 |
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+ | Splice-site prediction | Splicing (acceptor) | Cyprinidae (5 species) | 600 | 17200 / 2150 / 2150 |
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+ | Splice-site prediction | Splicing (donor) | Cyprinidae (5 species) | 600 | 27600 / 3450 / 3450 |
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+ | Splice-site prediction | Splicing (both) | Cyprinidae (5 species) | 600 | 1720 / 2150 / 2105 |
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+
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+ ## Data sources and construction overview
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+
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+ According to the FishNALM manuscript, FishGUE was assembled from the following sources:
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+
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+ - **Histone mark datasets** were derived from public zebrafish ChIP-seq peak datasets.
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+ - **Transcription factor binding-site datasets** were built from public ChIP-seq peaks for **CTCF**, **Pou5f1**, and **Sox2**.
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+ - **Promoter datasets** were constructed from zebrafish promoter annotations from the **Eukaryotic Promoter Database (EPD)**.
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+ - **Splice-site datasets** were compiled from annotated genomes of **five cyprinid fish species**.
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+
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+ For all tasks, positive and negative examples were constructed through task-specific processing, then split into **training**, **validation**, and **test** sets using an **8:1:1** ratio.
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+
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+ ## Suggested repository organization
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+
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+ If you plan to upload FishGUE as a single Hugging Face dataset repository, a clean structure is:
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+
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+ ```text
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+ FishGUE/
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+ ├── README.md
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+ ├── histone/
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+ │ ├── H3K4me1_train.tsv
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+ │ ├── H3K4me1_val.tsv
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+ │ ├── H3K4me1_test.tsv
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+ │ └── ...
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+ ├── tfbs/
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+ │ ├── CTCF_train.tsv
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+ │ ├── CTCF_val.tsv
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+ │ ├── CTCF_test.tsv
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+ │ └── ...
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+ ├── promoter/
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+ │ ├── core_promoter_300_train.tsv
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+ │ └── ...
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+ └── splice/
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+ ├── splice_acceptor_train.tsv
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+ └── ...
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+ ```
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+
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+ If your files are already packaged in another layout, you can keep that layout and simply explain it in this README.
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+
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+ ## Intended uses
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+
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+ FishGUE is intended for:
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+
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+ - benchmarking DNA foundation models on fish genomic sequence prediction tasks
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+ - evaluating transfer learning performance in fish genomics
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+ - comparing task robustness across promoter, chromatin, TFBS, and splice-site prediction settings
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+ - developing new fish-specific sequence models and downstream classifiers
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+
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+ ## Limitations
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+
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+ - Most non-splice tasks are based on **zebrafish** data.
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+ - The splice-site benchmark uses **five cyprinid species**, so broader phylogenetic coverage remains limited.
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+ - FishGUE is designed as a **research benchmark** and should not be interpreted as a clinical or diagnostic dataset.
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+ - Performance on FishGUE does not guarantee performance on all fish species or all regulatory genomics tasks.
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+
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+ ## Citation
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+
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+ If you use FishGUE in your work, please cite the FishNALM manuscript.
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+
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+ **Manuscript:**
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+
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+ > FishNALM: A Foundation DNA Language Model for Fish Genomes
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+
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+ ## Project links
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+
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+ - **GitHub**: [bioinfoihb/FishNALM](https://github.com/bioinfoihb/FishNALM)
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+
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+ ## Contact
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+
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+ **Xiao-Qin Xia**
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+ Institute of Hydrobiology, Chinese Academy of Sciences
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+ Email: xqxia@ihb.ac.cn
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+ Email: bioinfoihb@ihb.ac.cn