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Gunslinger's Saloon - Clearwater, FL | Groupon
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Ribs, duck, scallops, and steaks smoked in-house daily. Barbecue po'boys and fresh corn bread. Molasses-brined duck and USDA-certified steaks. The chefs at Gunslinger's Saloon combine old-fashioned barbecue with gourmet ingredients to create a menu of flavorful smoked dishes. Guests can chow down sweet potato fritters or a B.L.T. with house-cured bacon and fried green tomatoes on the patio bar, called The Sling, or can share a dish of the cobbler-of-the-day in the welcoming dining room.


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The Diamond Disco Society (Buffalo, NY)
get your tickets for Cupids Disco
http://www.sweetdealscumulus.com/deal/buffalo/classic-hits-1041-whtt-cupids-disco-party January 30 · 7:00 PM
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please RSVP on this site
Classic Hits 104.1's Spring Fling Disco Party
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The Forvm's photo.JoinSaturday Night FeverSaturday, February 7 at 7:00pmThe Forvm in Buffalo, New York3 people are going
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Patent US6562573 - Materials and methods for the purification of polyelectrolytes, particularly ... - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsThis invention describes materials and methods usable for the purification of polyelectrolytes, such as nucleic acids and proteins. The materials of the invention are separation media that possess pH-dependent groups with pKa value in the range of about 5 to about 7. Separation of the nucleic acids or...http://www.google.com/patents/US6562573?utm_source=gb-gplus-sharePatent US6562573 - Materials and methods for the purification of polyelectrolytes, particularly nucleic acidsAdvanced Patent SearchPublication numberUS6562573 B2Publication typeGrantApplication numberUS 09/840,570Publication dateMay 13, 2003Filing dateApr 23, 2001Priority dateJan 27, 1999Fee statusPaidAlso published asEP1147226A2, EP1147226A4, EP1147226B1, US20010041795, US20030204067, US20070117972, WO2000044928A2, WO2000044928A3Publication number09840570, 840570, US 6562573 B2, US 6562573B2, US-B2-6562573, US6562573 B2, US6562573B2InventorsFolim G. HalakaOriginal AssigneeFolim G. HalakaExport CitationBiBTeX, EndNote, RefManPatent Citations (6), Referenced by (15), Classifications (28), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetMaterials and methods for the purification of polyelectrolytes, particularly nucleic acidsUS 6562573 B2Abstract This invention describes materials and methods usable for the purification of polyelectrolytes, such as nucleic acids and proteins. The materials of the invention are separation media that possess pH-dependent groups with pKa value in the range of about 5 to about 7. Separation of the nucleic acids or proteins from a separation medium is effected at a neutral or higher pH.
CROSS-REFERENCE TO RELATED APPLICATIONS This application is a continuation-in-part of U.S. Ser. No. 09/238,343 filed on Jan. 27, 1999, which in turn is a continuation-in-part of U.S. Ser. No. 07/772,346 filed on Oct. 7, 1991, now abandoned.
FIELD OF THE INVENTION This invention relates to separation and purification of polyelectrolytes. In particular, the present invention relates to the purification of biochemical materials such as proteins and more particularly nucleic acids.
BACKGROUND OF THE INVENTION Purification of molecular species constitutes a crucial part to their production and utility. This is particularly important in the biotechnology and diagnostics fields. The present invention describes polymeric separation media and methods useful for the purification of molecular species that are polyelectrolytes, such as proteins and nucleic acids. The terms nucleic acid and polynucleotide are used interchangeably, and are used here to signify either a deoxyribonucleic acid (DNA) or a ribonucleic acid (RNA). Unless otherwise specified, the terms polynucleotide and oligonucleotide are used interchangeably.
COLPAN et al (DE patent number 4139664) describe a method for isolating nucleic acid from cells�by lysis of the cells, then elution of nucleic acid fixed to separation medium surface. However, the method recovers the nucleic acids by eluting with a buffer of high ionic strength.
HENCO et al (DE 3639949) describes a method for the Separation of long-chain nucleic acids�using a porous separation medium to fix the nucleic acids. The invention uses selective salt elution to first wash off the short chain nucleic acids whereas the long-chain nucleic acids are subsequently removed from the anion exchanger using a washing solution of high ionic strength.
Similar principles are offered by Bourque and Cohen (WO 9514087), where detection of charged oligonucleotides is accomplished by adsorbing on an ion exchange resin, eluting with a high salt buffer and detection. The oligonucleotides bind to the anion exchange resin at 40-65� C., while the desorbing from the resin with a high salt buffer is performed at 40-65�C.
NELSON et al (EP 281390) offered a method for the separation of small nucleotides from larger ones by binding to polycationic support�which does not retain smaller sequences for hybridization assays. The bound nucleic acids were apparently eluted, if needed, by 50% formamide, or other salts.
MACFARLANE (U.S. Pat. No. 5,300,635), uses quaternary amine surfactants�for isolating nucleic acids from a biological sample by forming complexes which can be dissociated.
HORNES and KORSNES (U.S. Pat. No. 5,512,439) also describe the detection and quantitative determination of target RNA or DNA�by contacting sample with magnetic particles carrying 5′-attached DNA probe.
SUMMARY OF THE INVENTION The present invention is directed to polymeric separation media and to methods useful for the purification of polyelectrolytes, particularly polynucleotides.
BRIEF DESCRIPTION OF THE DRAWINGS FIGS. 1(A) through 1(D) illustrate the formation of a polymeric medium over a period of time and embodying this invention. The medium is made by slowly adding water, 11, on top of a 2.5% (W/V) agarose solution in 8M urea, 12. A thin layer of gel, 13, which forms at the interface of the agarose and water layers, grows in thickness until all the agarose gels as time progresses from FIG. 1(A) to FIG. 1(D), and as described in Example 1;
FIG. 4(A) is a schematic of a polymeric separation medium, 41, provided with pendant groups, RH that are pH dependent. The polymeric separation medium may be one of polysaccharide, silica, or other polymers that can be functionalized to attach the groups. These are acid-base groups, and depending on the ambient pH value, the groups can be protonated, as shown (RH), or deprotonated (R). The groups can be a basic nitrogen, which can be protonated (�NH+) for polynucleotide purification, or the groups can be a deprotonated carboxyl (�COO−) or a deprotonated phenolic (�C6H4�O−) for the purification of positively charged proteins.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The polymeric separation media possess functional groups that are pH dependent, such as organic acids and organic bases. The pKa of the pH-dependent groups is chosen to be near or slightly below the neutral pH range, e.g., 5 to 7. For organic bases, usually containing nitrogen atoms, the pKa is that of the conjugated acid (base+proton). In the case of organic bases, if the pH is lowered to at or below the pKa, the bases acquire protons to form the conjugate acids. The separation medium thus acquires positive electrical charges, and bind negatively charged polyelectrolytes, such as nucleic acids and negatively charged proteins. The unbound solutes can be separated from the separation medium, and the separation medium can be washed with a low pH buffer. Raising the pH to a value above the pKa of the organic base will force the separation medium to loose its positive charges (protons), and thus release the bound nucleic acids in a pure form.
In another preferred embodiment, the above polymeric separation medium surrounds a magnetic core, such as paramagnetic iron oxide particles, as described schematically in FIG. 4(B). For example, magnetic particles may be coated by polymers, using techniques known in the art, to form magnetized separation media in particulate form. The polymer may be subsequently functionalized with pH-dependent groups as discussed herein. In another preferred method of purification, the sample containing the nucleic acids to be purified is equilibrated with the magnetized particles carrying basic groups at a pH near or slightly above the pKa of the groups (e.g., pKa=6, and pH�7), where they preferentially bind nucleic acids. Using a magnet, the particles are separated from the solution. The particles are washed with a buffer, also having a pH near the pKa of the basic groups and the particles are again separated using a magnet, and the wash buffer is aspirated. The bound nucleic acids may be eluted from the particles by a buffer having pH approximately 3 units higher than the pKa of the basic groups, thus releasing the nucleic acids in pure form.
In another preferred method of purification, the sample containing the nucleic acids to be purified is passed through polymeric separation medium containing basic groups, formed in the pores of the porous support. The process is carried out where the sample and the polymeric separation medium are equilibrated at a pH near or slightly above the pKa of the groups (e.g., pKa=6, and pH�7), whereby the nucleic acids bind to the polymeric separation medium. Wash buffer is then flown to wash off unbound solutes. Wash buffers may have the same pH as the binding buffer (�7). The bound nucleic acids may be eluted by a buffer having pH approximately 3 units higher than the pKa of the basic groups, thus releasing the nucleic acids in substantially pure form.
EXAMPLES Example 1 25 grams of agarose (Sigma Chemical Co., St. Louis, Mo., product number A9918) were dissolved in one liter of 8M urea solution by heating the solution with stirring until the solution became clear, forming layer 12, in FIG. 1. The medium is made by slowly adding water, 11, on top of a 2.5% (W/V) agarose solution in 8M urea, 12. A thin layer of gel, 13, which forms at the interface of the agarose and water layers, grows in thickness until all the agarose gels as time progresses from FIG. 1(A) to FIG. 1(D), and as described in Example 1. This solution remained ungelled after cooling to room temperature for a period of over 150 days. Upon slow addition of water, layer 11 in FIG. 1, to the top of this solution and standing unperturbed for a few hours, a gelled layer, 13, forms at the interface. This is indicated by the appearance of a white color. This layer plays an important role in preventing further mixing of the water with the agarose in the chaotropic solution. Water and urea molecules can cross this layer, but the agarose molecules, being considerably larger, remain in the bottom layer. At longer times, more urea molecules diffused to the top layer, until the whole bottom part became gelled, as shown in FIGS. 1(A) to 1(D). This solution behavior of agarose was repeated with quantities of agarose concentrations varying from 0.5 to 4% (in 8M urea solution). This solution behavior was also reproduced with 3M NaI solution and buffered 8M guanidine hydrochloride solution. The agarose dissolved to form a clear solution that did not gel at room temperature, however, it became yellowish with time in the case of NaI, presumably due to the oxidation of iodide ions. In the devices that were constructed using this approach, a gelled layer is formed at the surface of the porous support, preventing migration of the entrapped polymer from the pores. The gel structure of the entrapped polymer becomes more predictable as a result of this process.
Example 2 An ultra high molecular weight polyethylene (UHMWPE) porous cylinder, 9.5 mm O.D., 4.8 mm I.D., and 35 mm long was used, with one end sealed and the other open. This cylinder had nominal pore size of 20 microns and void volume (empty space in the walls of the cylinder) of approximately 50%. The inner bore of the cylinder was blocked by a rod of the same diameter as the ID of the cylinder to prevent the agarose solution from filling the bore. Agarose (2.5% w/v) was solubilized in 8M Urea as in Example 1. The agarose solution was vacuum drawn into the pores of the cylinder using a vacuum pump. The cylinder was soaked in water overnight. Referring to the schematic in FIG. 2, this cylinder is numbered 21. The agarose gelled in the pores of the cylinder as the urea diffused out to form the gel, 23. The rod was removed from the cylinder. A luer fitting attachment, 24, was sealed to the open end of the cylinder, and was used to attach the device to syringes or peristaltic pumps for the purpose of processing fluids. The device was placed in a casing, 22. The liquid permeability decreased from approximately 1800 for the gel-free device to about 8 after this step, indicating filling of the pores with the gel.
Example 3 The agarose-filled cylinder in Example 2 was equilibrated in 1M NaOH for 4 hours. The NaOH was drained out and the agarose was cross-linked using 3% epichlorohydrin in 95% (V/V) ethanol/water mixture for 16 hours at room temperature. The resulting cross-linked gel can be used a filtration device. The liquid permeability of the device after cross-linking increased slightly to about 10.
Example 4 The agarose in the cylinder of Examples 3 was allowed to react with 5% (W/V) p-(2-chloroethyl) pyridine (4-picolyl chloride) in 50% (W/V) reagent alcohol-water for 16 hour. This cylinder produced a device with a weak base that is suitable for DNA exchange, since the picolyl group has a pKa of about 6, it can bind DNA at neutral pH's and release DNA at moderately high pH (9).
Example 5 The agarose was cross-linked and re-equilibrated in 10% NaOH in a cylinder as in Examples 2-3. The cylinder was then allowed to react with 10% (W/V) chloroacetic acid in 95% (W/V) reagent alcohol-water for 16 hours. This cylinder produced a device with carboxyl group modified agarose as a weak cation exchanger.
Example 6 The cylinder in Example 5 was equilibrated with 15 mM Tris buffer at pH=7. Aliquots of 5 ml of cytochrome c at 1 mg/ml in the same buffer were loaded through the wall of the cylinder by closing one end of the cylinder. Total protein bound was 32 mg. The cylinder acquired the color of the protein. The device was washed with buffer, and the protein was eluted using aliquots of 500 mM buffered sodium chloride solution. As in Example 5, FIG. 8 shows the bind-elution histogram for cytochrome c.
Example 7 Sephadex G-50 beads (polysaccharide consisting of dextran and cross-linked agarose, Sigma Chemical Co., St, St. Louis, Mo.) were suspended in dioxane and allowed to react with 5% (W/V) p(2-chloroethyl) pyridine (4-picolyl chloride) hydrochloride in 95% (V/V) dioxane-water for 16 hours, with the addition of potassium carbonate as a base. The liquid was aspirated and the beads were washed repeatedly with water until the color disappeared. The beads were functionalized to produce a DNA purifier, since p-ethyl pyridine (gamma picoline) has a pKa of about 6, it can bind DNA at neutral pH's and release DNA at moderately high pH (9). As shown in FIG. 4, the polymeric separation medium may be one of polysaccharide, silica, or other polymers that can be functionalized to attach the groups. These are acid-base groups, and depending on the ambient pH value, the groups can be protonated, as shown (RH), or deprotonated (R). The groups can be a basic nitrogen, which can be protonated (�NH+) for polynucleotide purification, or the groups can be a deprotonated carboxyl (�COO−) or a deprotonated phenolic (�C6H4�O−) for the purification of positively charged proteins. FIG. 4(B) shows a schematic where the polymeric separation medium, 41, surrounds a magnetic core, 42, such as paramagnetic iron oxide.
Example 8 Approximately 50 mg of the beads in Example 7 were allowed to equilibrate with 0.3 ml of 30 mM acetate buffer of pH=5.5 in an eppendorf tube. 2 microgram solution of DNA (PBKSRV plasmid, from Strategene, �8 kb in length) in 0.05 ml of water were added to the beads and allowed to incubate at room temperature for approximately 10 minutes with mixing. The beads were centrifuged and the supernatant aspirated. The beads were washed three times with 0.5 ml each of the same buffer, centrifuged, and the buffer was aspirated after each wash. An aliquots of 0.2 ml of alkaline (pH=9) solution of HEPES buffer was added to the beads to elute the bound DNA, and the solution was aspirated. Another 0.5 ml elution step was applied, and again the supernatant was aspirated after centrifugation. After each aspiration step, the aspirated solution was saved for later DNA analysis by gel electrophoresis. Gel electrophoresis results indicate binding of the DNA under the acidic conditions and DNA release under the slightly alkaline elution.
Example 9 Paramagnetic iron oxide beads coated with silica were treated to produce a DNA purifier in a method similar to that in Example 7, by attaching 4-picoline to the silica separation medium.
Example 10 Approximately 50 mg of the magnetic beads in Example 9 were allowed to equilibrate with 0.3 ml of 30 mM acetate buffer of pH=5.5 in an eppendorf tube. 2 microgram solution of the DNA used in Example 8 in 0.05 ml of water were added to the beads and allowed to incubate at room temperature for approximately 10 minutes with mixing. A magnet was brought in contact with the tube, where the magnetic beads were attracted to the side of the tube contacting the magnet, and the supernatant aspirated. The beads were washed three times with 0.5 ml each of the same buffer, and after contacting the tube with the magnet, the buffer was aspirated after each wash. An aliquots of 0.2 ml of alkaline (pH=9) solution of HEPES buffer was added to the beads to elute the bound DNA, mixed and the supernatant aspirated after contacting the tube with the magnet. Another 0.5 ml elution step was applied, and again the supernatant was aspirated after magnetic separation. After each aspiration step, the aspirated solution was saved for analysis. Gel electrophoresis results indicated binding of the DNA under the acidic conditions and complete release of the DNA under the slightly alkaline elution.
Example 11 Approximately 50 mg of the magnetic beads in Example 9 were allowed to equilibrate with 0.5 ml of 30 mM acetate buffer of pH=5.5 in an eppendorf tube. 2 microgram solution of DNA in 0.2 ml of calf serum was added to the beads and the procedure in Example 10 was followed. Gel electrophoresis results indicate binding of the DNA under the acidic conditions and complete release of the DNA under the slightly alkaline elution. The released DNA band in this example was slightly diffuse, presumably due to DNA degradation by the action of nucleases present in the serum, a condition that can be avoided by the addition of a protease or other nuclease-deactivating substances.
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS4335017Dec 8, 1977Jun 15, 1982United Kingdom Atomic Energy AuthorityComposite materials comprising deformable xerogel within the pores of particulate rigid supports useful in chromatographyUS4724207Aug 22, 1984Feb 9, 1988Cuno IncorporatedModified siliceous chromatographic supportsUS4752459Mar 27, 1986Jun 21, 1988Perrer Duncan SPreparation of porous bodiesUS5019232Jun 1, 1990May 28, 1991Minnesota Mining And Manufacturing CompanyMedium for electrophoresisUS5581349Mar 27, 1995Dec 3, 1996Halaka; Folim G.Method for biological cell and particulate analysisUS5876937 *Jul 1, 1996Mar 2, 1999Akzo Nobel N.V.Method for determining the integrity of nucleic acid* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS6914137Dec 14, 2000Jul 5, 2005Dna Research Innovations LimitedIsolation of nucleic acidsUS7319004Jun 2, 2003Jan 15, 2008Invitrogen CorporationMaterials and methods relating to polyions and substance deliveryUS7479222Feb 3, 2005Jan 20, 2009Millipore CorporationPorous adsorptive or chromatographic mediaUS7479223Feb 3, 2005Jan 20, 2009Millipore CorporationPorous adsorptive or chromatographic mediaUS7687619Feb 3, 2005Mar 30, 2010Millipore CorporationRoom temperature stable agarose solutionsUS7807823Feb 3, 2005Oct 5, 2010Millipore CorporationMethod of forming polysaccharide structuresUS7824548Jun 21, 2007Nov 2, 2010Millipore CorporationPorous adsorptive or chromatographic mediaUS7959979Feb 3, 2005Jun 14, 2011Millipore CorporationMethod of forming coated structuresUS8008476Mar 26, 2010Aug 30, 2011Millipore CorporationRoom temperature stable agarose solutionsUS8137561Jun 23, 2011Mar 20, 2012Emd Millipore CorporationMedia for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the sameUS8158196Jun 14, 2011Apr 17, 2012Emd Millipore CorporationMethod of forming coated structuresUS8298657May 5, 2011Oct 30, 2012Alex Garfield BonnerPorous interpenetrating polymer networkUS8415467Dec 13, 2010Apr 9, 2013Betty WuMethod and materials for separating nucleic acid materialsUS8435406Feb 1, 2012May 7, 2013Emd Millipore CorporationMedia for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the sameUS8552177Aug 30, 2011Oct 8, 2013Emd Millipore CorporationRoom temperature stable agarose solutionsClassifications U.S. Classification435/6.16, 536/24.33, 536/24.31, 435/91.1, 585/800International ClassificationC07K1/18Cooperative ClassificationC08L5/12, C12N15/1006, B01J20/26, B01J20/265, B01J39/185, B01J41/125, B01J20/262, B01J20/28009, B01J20/28097, C07K1/18, C08B37/0039European ClassificationC08L5/12, B01J41/12B, B01J20/26E, B01J20/28B8, B01J20/28F20B, C08B37/00M4B, B01J39/18B, B01J20/26, C12N15/10A2, B01J20/26K, C07K1/18Legal EventsDateCodeEventDescriptionNov 15, 2010FPAYFee paymentYear of fee payment: 8Feb 13, 2009SULPSurcharge for late paymentFeb 13, 2009FPAYFee paymentYear of fee payment: 4Feb 9, 2009PRDPPatent reinstated due to the acceptance of a late maintenance feeEffective date: 20090213May 13, 2007REINReinstatement after maintenance fee payment confirmedNov 29, 2006REMIMaintenance fee reminder mailedRotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services©2012 Google

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Patent US7879027 - Controlled release devices for fusion of osteal structures - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsA device includes a first reservoir configured to include a degradation agent, a first control element configured to provide access to the first reservoir, and an expandable component configured to extend to engage an osteal structure....http://www.google.com/patents/US7879027?utm_source=gb-gplus-sharePatent US7879027 - Controlled release devices for fusion of osteal structuresAdvanced Patent SearchPublication numberUS7879027 B2Publication typeGrantApplication numberUS 11/517,771Publication dateFeb 1, 2011Filing dateSep 8, 2006Priority dateApr 24, 2006Fee statusPaidAlso published asUS20070276337, WO2008030832A1Publication number11517771, 517771, US 7879027 B2, US 7879027B2, US-B2-7879027, US7879027 B2, US7879027B2InventorsHai H. TrieuOriginal AssigneeWarsaw Orthopedic, Inc.Export CitationBiBTeX, EndNote, RefManPatent Citations (74), Non-Patent Citations (14), Referenced by (2), Classifications (27), Legal Events (3) External Links: USPTO, USPTO Assignment, EspacenetControlled release devices for fusion of osteal structures
In addition, the implantable device can include an anti-inflammatory agent. An exemplary anti-inflammatory agent can include a soluble tumor necrosis factor α-receptor, a pegylated soluble tumor necrosis factor α-receptor, a monoclonal antibody, a polyclonal antibody, an antibody fragment, a COX-2 inhibitor, a metalloprotease inhibitor, a glutamate antagonist, a glial cell derived neurotrophic factor, a B2 receptor antagonist, a substance P receptor (NK1) antagonist, a downstream regulatory element antagonistic modulator (DREAM), iNOS, an inhibitor of tetrodotoxin (TTX)-resistant Na+-channel receptor subtypes PN3 and SNS2, an inhibitor of interleukin, a TNF binding protein, a dominant-negative TNF variant, Nanobodies™, a kinase inhibitor, or any combination thereof. Another exemplary anti-inflammatory agent can include Adalimumab, Infliximab, Etanercept, Pegsunercept (PEG sTNF-R1), Onercept, Kineret�, sTNF-R1, CDP-870, CDP-571, CNI-1493, RDP58, ISIS 104838, 1→3-β-D-glucan, Lenercept, PEG-sTNFRII Fc Mutein, D2E7, Afelimomab, AMG 108, 6-methoxy-2-napthylacetic acid or betamethasone, capsaiein, civanide, TNFRc, ISIS2302 and GI 129471, integrin antagonist, alpha-4 beta-7 integrin antagonist, cell adhesion inhibitor, interferon gamma antagonist, CTLA4-Ig agonist/antagonist (BMS-188667), CD40 ligand antagonist, Humanized anti-IL-6 mAb (MRA, Tocilizumab, Chugai), HMGB-1 mAb (Critical Therapeutics Inc.), anti-IL2R antibody (daclizumab, basilicimab), ABX (anti IL-8 antibody), recombinant human IL-1 0, HuMax IL-15 (anti-IL 15 antibody), or any combination thereof.
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Accessed May 14, 2009.2Bron JL et al. 2009. Repair, regenerative and supportive therapies of the annulus fibrosus: achievements and challenges. Eur Spine J 18:301-313.3Chymopapain from GenBank Accession No. CAA66378, pp. 1-2. Accessed May 14, 2009.4Collagenase from GenBank Accession No. CAA07432, pp. 1-3. Accessed May 14, 2009.5Fibroblast growth factor from GenBank Accession No. CAA41788, pp. 1-2. Accessed May 14, 2009.6Morphogenetic protein from GenBank Accession No. NP-391488, pp. 1-3, Accessed May 14, 2009.7Morphogenetic protein from GenBank Accession No. NP—391488, pp. 1-3, Accessed May 14, 2009.8NCBI; "Albumin [Homo Sapiens]": CAA00606; http:www.nebi.nlm.nih.gov/protein/412163; printed on May 14, 2009; 2 pages.9NCBI; "Chymopapain [Carica Papaya]"; CAA66378; http:www.nebi.nlm.nih.gov/protein/1332461; printed on May 14, 2009; 2 pages.10NCBI; "Collagenase [Rattus Norvegicus]", CAA07432; http:www.nebi.nlm.nih.gov/protein/3242321; printed on May 14, 2009; 3 pages.11NCBI; "Fibroblast Growth Factor [Homo Sapiens]"; CAA41788; http:www.nebi.nlm.nih.gov/protein/1335059; printed on May 14, 2009; 2 pages.12NCBI; "Morphogenetic Protein [Bacillus Subtilis Subsp. Subtilis Str. 168]"; NP-39148l http:www.nebi.nlm.nih.gov/protein/16080660, printed on May 14, 2009; 3 pages.13NCBI; "Morphogenetic Protein [Bacillus Subtilis Subsp. Subtilis Str. 168]"; NP—39148l http:www.nebi.nlm.nih.gov/protein/16080660, printed on May 14, 2009; 3 pages.14Sheikh H et al. 2009. In vivo intervertebral disc regeneration using stem cell-derived chondroprogenitors. J Neurosurg Spine 10: 265-272.Referenced byCiting PatentFiling datePublication dateApplicantTitleUS9289584Sep 13, 2011Mar 22, 2016The University Of British ColumbiaRemotely controlled drug delivery systemsUS20120029638 *Jul 30, 2010Feb 2, 2012Warsaw Orthopedic, Inc.Vertebral body replacement device configured to deliver a therapeutic substance* Cited by examinerClassifications U.S. Classification604/892.1, 604/891.1, 623/17.12, 604/66, 604/65International ClassificationA61K9/22Cooperative ClassificationA61B2017/0256, A61B17/58, A61K9/0004, A61M2005/14513, A61B2017/00557, A61M2210/02, A61B17/7061, A61F2002/30677, A61F2002/3068, A61M5/14276, A61B17/8858, A61F2250/0068, A61B17/7097, A61F2002/2817, A61M5/16827, A61M2210/1003European ClassificationA61M5/142P10, A61K9/00L4, A61B17/58, A61B17/70L, A61B17/88C2DLegal EventsDateCodeEventDescriptionSep 8, 2006ASAssignmentOwner name: WARSAW ORTHOPEDIC, INC., INDIANAFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:TRIEU, HAI H.;REEL/FRAME:018298/0027Effective date: 20060906Apr 5, 2011CCCertificate of correctionAug 1, 2014FPAYFee paymentYear of fee payment: 4RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services


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Best Deals for Liki Loft Hotel, Saint Petersburg, Russia - Booking.com
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value: This website is a Dutch online travel agency for lodging reservations & other travel products, and a subsidiary of Booking Holdings. It is headquartered in Amsterdam.The website has over 28 million listings.


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The dueling plans were a departure from the so-called grand bargain that Mr. Obama had been pushing, which would have included trillions of dollars in budget and entitlement cuts over the next 10 years along with the elimination of tax loopholes and possibly the addition of new taxes.The impasse has set the tone for the 2012 presidential race, with the debate growing rancorous and the two parties’ visions for the country at odds.
The enormous deficit challenges have been clearly articulated to voters, as the parties seek unified control of Congress, where the Republicans are the majority in the House and the Democrats in the Senate. Mr. Obama will veto any debt legislation unless it extends the ability of the nation to borrow into 2013, the White House chief of staff, William M. Daley, said on Sunday.Mr. Daley, appearing on the NBC News program “Meet the Press,” said that world markets and the American economy would not tolerate continued doubts brought on by periodic fights over the debt ceiling. “The president believes that we must get this uncertainty out of the system,” Mr. Daley said.
Both Mr. Geithner and Mr. Daley said they believed Congress would figure out a way to avoid default.“The leaders of Congress have said unequivocally that we will meet our obligations,” Mr. Geithner said.
Mr. Daley acknowledged, however, that both sides were still at the brink.“We are now getting to a point where markets around the world will question whether the political system can come together and compromise for the good of the country,” Mr. Daley said.

value: This website is an American daily newspaper based in New York City with a worldwide readership. Founded in 1851, the Times has since won 132 Pulitzer Prizes, the most of any newspaper, and has long been regarded within the industry as a national "newspaper of record".


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Rise Up Times (riseuptimes) on Pinterest
Check Rise Up Times for news, information, stories, commentary, videos and perceptive views on current events and media issues.
Surveillance, Freedom of Speech, Civil Rights and Liberties
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Unrigging The System: Reformers, Civic Groups Take Action Following Historic Summit. The Unrig The System Summit in New Orleans was a historic event that brought together people from across the political spectrum who could all agree on at least one thing: Our political process is corrupt, rigged, and need of broad systemic reform.
Latin America is described as the back yard of the United States. There is a long history of US intervention in Latin American countries and vibrant resistance both to the US and to vicious right wing governments. Current hot spots are Venezuela, where the US is now openly advocating regime change, Colombia, which is struggling to live up to its peace agreement, and the Honduras, where the new president is being heavily protested after a fraudulent election.
UK courts beginning to back public opposition to weapons exports to repressive regimes. More than 100 were arrested while protesting weapons sales to worlds worst rights abusers.
The Teflon Toxin Part 15 The U.S. military is spending billions to clean up drinking water contaminated with toxic firefighting foam while continuing to use dangerous new formulas. James Heidinger poses for a portrait while sitting on his contaminated well on his land in Coupeville on Whidbey Island, Washington on July 13, 2017.

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scubstev834 comments on My gf and I just rescued this little gal. She's a Cane Corso Mastiffjump to contentmy subredditsannouncementsArtAskRedditaskscienceawwblogbookscreepydataisbeautifulDIYDocumentariesEarthPornexplainlikeimfiveFitnessfoodfunnyFuturologygadgetsgamingGetMotivatedgifshistoryIAmAInternetIsBeautifulJokesLifeProTipslistentothismildlyinterestingmoviesMusicnewsnosleepnottheonionOldSchoolCoolpersonalfinancephilosophyphotoshopbattlespicsscienceShowerthoughtsspacesportstelevisiontifutodayilearnedTwoXChromosomesUpliftingNewsvideosworldnewsWritingPromptsedit subscriptionsfront-all-random | LifeProTips-television-funny-AskReddit-UpliftingNews-science-nosleep-Jokes-tifu-Fitness-dataisbeautiful-Music-personalfinance-gaming-IAmA-pics-worldnews-aww-Documentaries-videos-gifs-creepy-sports-food-books-DIY-news-Art-Showerthoughts-mildlyinteresting-GetMotivated-nottheonion-InternetIsBeautiful-TwoXChromosomes-gadgets-movies-philosophy-OldSchoolCool-askscience-EarthPorn-Futurology-WritingPrompts-todayilearned-photoshopbattles-explainlikeimfive-listentothis-history-spacemore » awwcommentsrelatedwant to join? sign in or create an account in seconds|Englishlimit my search to /r/awwuse the following search parameters to narrow your results:subreddit:subredditfind submissions in "subreddit"author:usernamefind submissions by "username"site:example.comfind submissions from "example.com"url:textsearch for "text" in urlselftext:textsearch for "text" in self post contentsself:yes (or self:no)include (or exclude) self postsnsfw:yes (or nsfw:no)include (or exclude) results marked as NSFWe.g. subreddit:aww site:imgur.com dogsee the search faq for details.advanced search: by author, subreddit...this post was submitted on 07 Mar 2011333 points (92% upvoted)shortlink: remember mereset passwordloginSubmit a new linkSubmit a new text postawwunsubscribesubscribe6,113,787 readers4,583 users here nowIf you don't have our CSS enabled or are on mobile, our sidebar will look weird! Sorry for the mess!
a community for 7 yearsmessage the moderatorsMODERATORScarelessmoomookittyMrsRattPenguinTransportLivyka316nutsabsurdlyobfuscatedRicoVigParakeetNipple...and 9 more »332333334My gf and I just rescued this little gal. She's a Cane Corso Mastiff (i.imgur.com)submitted 3 years ago by scubstev83489 commentssharecancelsorry, this has been archived and can no longer be voted onloading...sorted by: bestbesttopnewhotcontroversialoldrandomyou are viewing a single comment's thread.view the rest of the comments →[–]scubstev834[S] 4 points5 points6 points 3 years ago (1 child)sorry, this has been archived and can no longer be voted onDefinitely not. Its actually set up through the Delaware Puppy Rescue. You volunteer to either foster or adopt. We chose adoption! permalinkparent[–]Barbaro 1 point2 points3 points 3 years ago (0 children)sorry, this has been archived and can no longer be voted onYay! Awesome! Here's hoping you enjoy her for many long years. (I recently lost my first adoptee after thirteen years. The whole first year was a challenge, but she mellowed into the best. dog. ever.)
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9781592217373: A Tale of Three Women, a novel - AbeBooks - Eustace Palmer: 1592217370
Eustace Palmer A Tale of Three Women, a novel
ISBN 13: 9781592217373
A Tale of Three Women, a novel
ISBN 10: 1592217370 ISBN 13: 9781592217373
Publisher: Africa World Press, 2011
Set in Sierra Leone, West Africa, A Tale of Three Women is epic in scope , covering a span of about sixty years and touching upon the most important developments in that country s recent history from about 1918 to the 1970s. It encompasses events such as the worldwide influenza epidemic, the Second World War, the preparation for independence, the achievement of independence, and the post-independence malaise. But all these serve only as a background against which Eustace Palmer deftly weaves the experiences of three very different women who were originally friends at a traditional sewing school, but whose fortunes take widely different turns through their different characters and the choices they and others make. In the process the novel becomes a penetrating study of the condition of women women whose husbands are chosen for them; women who lack opportunity for advancement in a male-dominated world; women who are exploited, abused, and betrayed by men; women who are also capable of oppressing and betraying each other; and women who are capable of standing on their own and making it in spite of men. This is a tale about friendship, class-consciousness and snobbery, betrayal, treachery, love, hatred, and death a rich fabric into which the author cleverly weaves the customs and traditions of the Creoles of Sierra Leone s Western area, such as the ceremonies that accompany the various rites of passage like birth, weddings and funerals. The subtle delineation of character and evocation of atmosphere make this densely textured but eminently readable work a fascinating tale.
A native Sierra Leonean, Eustace Palmer, one of the pioneer critics of African literature, has published one book on the English novel Studies in the English Novel and four on African literature: An Introduction to the African Novel; The Growth of the African Novel; Of War and Women, Oppression and Optimism: New Essays on the African Novel; and Knowledge Is More Than Mere Words: A Critical Introduction to Sierra Leonean Literature (jointly edited with Abioseh Michael Porter). He has also published over sixty articles on English and African literatures. For several years, he was Associate Editor of African Literature Today and was President of the African Literature Association from 2006 to 2007. A Tale of Three Women is his first published novel. He currently teaches at Georgia College & State University where he is Professor of English and Coordinator of Africana Studies.
1. A Tale Of Three Women (Paperback)
Published by Africa Research Publications, United States (2011)
Book Description Africa Research Publications, United States, 2011. Paperback. Book Condition: New. Language: English . Brand New Book. Bookseller Inventory # AAT9781592217373
3. A Tale of Three Women, a novel
Book Description Africa World Press, 2011. Paperback. Book Condition: New. book. Bookseller Inventory # M1592217370
4. A Tale of Three Women, a novel
Book Description Paperback. Book Condition: New. Brand New! We ship daily Monday - Friday!. Bookseller Inventory # 1EY82M0072EW
5. Tale of Three Women, A
Book Description AFRICA WORLD PRESS. Paperback. Book Condition: New. New copy - Usually dispatched within 2 working days. Bookseller Inventory # B9781592217373
Published by Africa Research and Publications (2011)
Book Description Africa Research and Publications, 2011. PAP. Book Condition: New. New Book. Shipped from UK in 4 to 14 days. Established seller since 2000. Bookseller Inventory # EB-9781592217373
7. A Tale of Three Women, a novel
Book Description Africa World Press, 2011. Paperback. Book Condition: Brand New. first edition edition. 236 pages. 8.20x5.30x0.70 inches. In Stock. Bookseller Inventory # __1592217370
8. A Tale of Three Women, a novel
Book Description Africa World Press, 2011. Paperback. Book Condition: New. Never used!. Bookseller Inventory # P111592217370

value: This website is an e-commerce global online marketplace with seven websites that offer books, fine art, and collectables from sellers in over 50 countries. Launched in 1996, it specialises in used, rare and out-of-print books.


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Electric Contractors Commercial Industrial in College Village Anchorage, Alaska with Reviews & Ratings - YP.comFind People•Advertise With UsBrowseFind PeopleFeatured CollectionsRestaurantsAutomotiveHome ImprovementHealth & WellnessAttorneysAdvertise with usWhat do you want to find?Search by business name, or keywordnearWhere? 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Raven Electric IncServices(17)BBB Rating: A+8015 Schoon StAnchorage, AK 99518(907) 349-9668Electric Contractors-Commercial...WebsiteYP AdDirectionsMore InfoRaven Electric have done both commercial and residential work for me. Their work completely met my expectations.Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection2. Stusser Electric CoServicesBBB Rating: A+411 E 54th AveAnchorage, AK 99518(907) 561-1061Electric Contractors-Commercial...WebsiteDirectionsMore InfoFrom Business: Stusser Electric Co. is located at 411 E. 54th Ave here in Anchorage Alaska. We have stocked inventory at that location since 1984. We have access to most major bra…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection3. Shelton ElectricBBB Rating: A+1720 E 59th AveAnchorage, AK 99507(907) 563-3249Electric Contractors-Commercial...WebsiteYP AdDirectionsMore InfoFrom Business: Commercial, ResidentialAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection4. Supreme ElectricServicesBBB Rating: A+1301 W 42nd Ave # 3Anchorage, AK 99503(907) 561-7776Electric Contractors-Commercial...WebsiteDirectionsMore InfoFrom Business: Supreme Electric started out as a two man team. Justin and Keith began a small-time electrical company that worked with some companies around town. After five short…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection5. Megawatt ElectricServicesBBB Rating: A+510 W Fireweed LnAnchorage, AK 99503(907) 277-6002Electric Contractors-Commercial...WebsiteFrom Business: Founded in 2004, Megawatt Electric is an electrical contracting firm. The company employs more than 50 people, as well as offers engineering services. It offers job…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection6. National Electrical Contractors Association Alaska Chapter712 W 36th AveAnchorage, AK 99503(907) 561-1958Electric Contractors-Commercial...WebsiteYP AdDirectionsMore InfoFrom Business: National electrical contractor's associate Alaska chapter has been serving Alaska electrical contracting needs since before statehood. Specialize in commercial, ind…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection7. Chariot Group IncBBB Rating: A+3120 Denali St Ste 1Anchorage, AK 99503(907) 222-5300Electric Contractors-Commercial...WebsiteFrom Business: The Chariot Group is an information technology company that offers a range of videoconference and multimedia solutions. The group provides a variety of consulting a…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection8. C2C ElectricBBB Rating: A+3705 Arctic Blvd Suite 665Anchorage, AK 99503(907) 248-3925Electric Contractors-Commercial...WebsiteYP AdDirectionsMore InfoFrom Business: Electrical contractors specializing in small jobs / Residential and Commercial services. Call for an appointment or consultation.Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection9. Samson Electric Inc129 W Potter DrAnchorage, AK 99518(907) 336-1258Electric Contractors-Commercial...WebsiteYP AdDirectionsMore InfoAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection10. All Wire ElectricBBB Rating: A+140 E 45th AveAnchorage, AK 99503(907) 339-9473Electric Contractors-Commercial...From Business: ,Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection11. Knik ElectricBBB Rating: A+2002 N Post RdAnchorage, AK 99501(907) 272-7374Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection12. AC ElectricalBBB Rating: A+PO Box 140792Anchorage, AK 99514(907) 632-1629Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection13. Aleyeska Electric Contracting4134 Ingra St # BAnchorage, AK 99503(907) 770-7913Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection14. Progressive Electric Group1147 E Dowling RdAnchorage, AK 99518(907) 891-2708Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection15. Inlet Electrical Contractors201 Post RdAnchorage, AK 99501(907) 272-2004Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection16. Inlet Electrical Contractors1565 Bragaw St Ste 207Anchorage, AK 99508(907) 272-2004Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection17. M & M Electric IncAnchorage, AK 99522(907) 522-6609Electric Contractors-Commercial...WebsiteYP AdMore InfoFrom Business: We are professionals in the electrical business and guarantee the best for our clients. Our licensed and certified experts provide the finest and most reliable elec…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection18. Glacier ElectricBBB Rating: A+3831 Crosson CirAnchorage, AK 99517(907) 306-1071Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection19. Northern Powerline ConstructorBBB Rating: A+775 E 100th AveAnchorage, AK 99515(907) 344-3436Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection20. Talon ElectricBBB Rating: A+11430 Hawkins LnAnchorage, AK 99516(907) 561-1585Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection21. Errico Electrical EngineeringBBB Rating: A+Anchorage, AK 99522(907) 345-6168Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection22. Industrial Commercial Electric Inc384 Eklutna StAnchorage, AK 99504(907) 338-9714Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection23. Glacier Electric3410 Eastwind DrAnchorage, AK 99516(907) 306-1071Electric Contractors-Commercial...WebsiteYP AdDirectionsMore InfoFrom Business: Ad Licensed Bonded and Insured. Do it all for Residential and commercial buildings with custom residential as a specialty.Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection24. Electric & MoreBBB Rating: A+4700 Riverton AveAnchorage, AK 99516(907) 345-4530Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection25. Laker Electric IncServices11901 Industry Way Ste A11Anchorage, AK 99515(907) 677-7886Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection26. Need Electrical Contractors5221 E 131st AveAnchorage, AK 99516(907) 345-8936Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection27. Northwest Electrical SVCBBB Rating: A+17420 Golden View DrAnchorage, AK 99516(907) 336-3539Electric Contractors-Commercial...Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection28. Quality EnterprisesBBB Rating: A+20433 Lucas AveEagle River, AK 99577(907) 223-0673Electric Contractors-Commercial...WebsiteAdd to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection29. Prime Constructors LLC2231 N Northway Ln Unit BPalmer, AK 99645(907) 240-0319Electric Contractors-Commercial...WebsiteDirectionsMore InfoFrom Business: Prime Constructors LLC is a General Contracting Company including our own Electrical outfit that provides quality ground up through finish services in Commercial an…Add to mybookRemove from mybookAdded to your home collection!Error when adding to home collectionThis business was removed from the home collection30. AVID Audio / Video23405 Hilltop DrChugiak, AK 99567(907) 688-9473Electric Contractors-Commercial...YP AdDirectionsMore InfoSponsored LinksShowing1-30
of 107results1234NextAdd a New CollectionOops! There was a problem saving the new custom collection.Please try again.Collection Name cannot be emptyUpload a Photo(optional)Remaining Characters: 100Remaining Characters: 500CancelSaveMap ViewFeatured Electric Contractors-Commercial & Industrialin College VillageRaven Electric Inc(17)8015 Schoon St, Anchorage, AK 99518(907) 349-9668WebsiteYP AdDirectionsMore InfoStusser Electric Co411 E 54th Ave, Anchorage, AK 99518(907) 561-1061WebsiteDirectionsMore InfoSupreme Electric1301 W 42nd Ave # 3, Anchorage, AK 99503(907) 561-7776WebsiteDirectionsMore InfoSamson Electric Inc129 W Potter Dr, Anchorage, AK 99518(907) 336-1258Quality Service When It Counts.WebsiteYP AdDirectionsMore InfoNational Electrical Contractors Association Alaska Chapter712 W 36th Ave, Anchorage, AK 99503(907) 561-1958WebsiteYP AdDirectionsMore InfoM & M Electric IncAnchorage, AK 99522(907) 522-6609WebsiteYP AdMore InfoGlacier Electric3410 Eastwind Dr, Anchorage, AK 99516(907) 306-1071WebsiteYP AdDirectionsMore InfoAVID Audio / Video23405 Hilltop Dr, Chugiak, AK 99567(907) 688-9473YP AdDirectionsMore InfoC2C Electric3705 Arctic Blvd Suite 665, Anchorage, AK 99503(907) 248-3925WebsiteYP AdDirectionsMore InfoPrime Constructors LLC2231 N Northway Ln Unit B, Palmer, AK 99645(907) 240-0319WebsiteDirectionsMore InfoShelton Electric1720 E 59th Ave, Anchorage, AK 99507(907) 563-3249"One Of Anchorages Most Experienced Electric Contractors Since 1975"WebsiteYP AdDirectionsMore InfoDidn't find what you were looking for?magnifying glassPlease help others by helping us do better.Suggest a BusinessHelpful Reviews Raven Electric Incak4me1 ratedOur telecommunications departm...Our telecommunications department has been working closely with Raven Electric for about 2 years now. They have developed a cabling system specifically for our company that has proven extremely efficient and effective. I definitely recommend them and it is only a bonus that they are a pleasure to work withRaven Electric IncJennie H. ratedMy family has been working with Raven Electric for over 30 years now, and feel as if they are an excellent company. They're extremely reliable, and resolve problems quickly. Raven Electric IncRachel C. ratedThe service technician that we used knew a ton about what were doing with the lighting and what the problems were around the house. I was very satisfied with their work. AboutAbout UsSite FeedbackContact UsAdvertise with UsCareers - We're HiringCorporate BlogEngineering BlogLegal

value: This website is a United States-based web site operated by YP Holdings that provides listings for local businesses. In 2013 it was re-branded as YP.com or simply "YP".