However, in the same year as the International Society of Protistologists' classification was published (2005), doubts were being expressed as to whether some of these supergroups were monophyletic, particularly the Chromalveolata, and a review in 2006 noted the lack of evidence for several of the six proposed supergroups.
Men, samma år som protologistsällskapets klassifikation publicerades (2005), uttrycktes tvivel på huruvida några av supergrupperna var monofyletiska, speciellt gällde detta Chromoalveolata, och en genomgång 2006 påpekade bristen på bevis för flera av de sex grupperna..