Next in line after the king's children was his sister Margaret Tudor, and her children, but their place in the succession was not secure – Henry would later exclude them by the Second Succession Act (1536), and by his will.
Närmast efter kungens barn i tronföljden stod hans syster Margaret Tudor och hennes barn, men deras plats var inte säker - Henrik skulle senare utesluta dem genom den andra successionsakten (1536) och i sitt testamente.