With increased use of the subways compared to the elevated lines, and the completion of the unification of the city's three subway systems, the Fifth Avenue Line was closed at midnight on June 1, 1940, and was demolished in 1941.
由於相比高架鐵路越來越多的人使用地鐵，在城市三個地鐵系統完成統一以後，第五大道線於1940年6月1日關閉，並於1941年拆除。